Effects of Blue Light Filtration on Photostress-Induced Retinal Damage in Rats

Hikage F, Hiraoka M, Ookawa E, Abe A, Ohguro H

Background: An eye with intraocular lens (IOL) implanted is easier to have retinal damage caused by blue light exposure, when the lens is removal. The aim of the present study is to evaluate retinal morphology and photoreceptor functions including rhodopsin (Rho) regeneration and Rho phosphorylation and dephosphorylation in rats subjected to photostress in the presence or absence of a yellow filter that effectively reduces blue light. Methods: We exposed rats to a 2500 or 5000 lux photostress for 24 hours in a photostress box with or without a yellow filter. After the treatment, we evaluated retinal morphology and function by electroretinogram (ERG). To examine photoreceptor function, during dark adaptation following the photostress, we employed a spectrometric assay to quantify Rho regeneration, and immunohistochemistry to evaluate in vivo Rho phosphorylation and dephosphorylation at 334Ser or 338Ser. Results: Levels of Rho bleaching upon photostress, and rates of Rho regeneration and Rho dephosphorylation during dark adaptation following the photostress were comparable with or without the yellow filter. However, retinal morphology and ERG responses were significantly preserved by the filter in both 2500 lux and 5000 lux photostresses. Conclusions: Our study reveals that although there was little impact on photostress-induced changes in photoreceptor function, reducing blue light effectively reduced photostress-induced retinal damage.

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